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2 edition of Gene expression profiling of a CpG-ODN-dependent antiviral system in RAW 264.7 cells. found in the catalog.

Gene expression profiling of a CpG-ODN-dependent antiviral system in RAW 264.7 cells.

Michael Litvack

Gene expression profiling of a CpG-ODN-dependent antiviral system in RAW 264.7 cells.

by Michael Litvack

  • 177 Want to read
  • 23 Currently reading

Published .
Written in English


About the Edition

CpG-oligodeoxynucleotides (CpG-ODN) confer local antiviral properties when delivered to the genital mucosa of mice. CpG-ODN treatment also prevents the replication of the herpes simplex virus 2 (HSV-2) in the murine macrophage cell-line RAW 264.7. We hypothesized that this CpG-dependent antiviral system is the result of alterations in gene expression. In this study we used high density cDNA and oligonucleotide microarrays to survey the gene expression of the mouse genome over seven time points within a 24h period in order to elucidate a program of genes responsible for the antiviral effect. We used hierarchical and/or K-means clustering to reveal similarly regulated genes. Interferon-related genes including Stat-1 and -2, IRF-3 and -7, and several members of the powerful antiviral oligoadenylate synthetase (OAS) family were regulated by CpG-ODN. We also inhibited NFkappaB activation and showed that CpG-dependent cytokine secretion requires NFkappaB, but that the antiviral effect persists independently of NFkappaB activity.

The Physical Object
Pagination150 leaves.
Number of Pages150
ID Numbers
Open LibraryOL19215207M
ISBN 100494022655

Gene expression profiling in aggressive digital papillary adenocarcinoma sheds light on the architecture of a rare sweat gland carcinoma. H.M. Surowy. Heinrich‐Heine‐University, Medical Faculty, Institute of Human Genetics, Düsseldorf, Germany. Search for more papers by this author. Clinical and Diagnostic Laboratory Immunology, ISSN X, 05/, Vol Issue 3, pp. -

Purpose: Although the central role of the immune system for tumor prognosis is generally accepted, a single robust marker is not yet available. Experimental Design: On the basis of receiver operating characteristic analyses, robust markers were identified from a gene B cell–derived metagene and analyzed in gene expression profiles of 1, breast cancer; 1, non–small cell lung. A versatile viral system for expression and depletion of proteins in mammalian cells. Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, Campisi J, Yaswen P, Cooper PK, Kaufman PD. PLoS One. Aug 6;4(8):e PubMed Article.

Abstract. Cardiotoxicity is one of the leading causes of drug attrition. Current in vitro models insufficiently predict cardiotoxicity, and there is a need for alternative physiologically relevant models. Here we describe the gene expression profile of human-induced pluripotent stem cell–derived cardiocytes (iCC) postthaw over a period of 42 days in culture and compare this profile to human. Gene Expression Profiling. Identifying Cell-Specific Transcripts More is known about the cellular architecture and development of C. elegans than of any other metazoan. For example, the lineal origin and morphology of all somatic cells are fully established. In addition, a wiring diagram traces every synapse among the neurons in the worm nervous system.


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Gene expression profiling of a CpG-ODN-dependent antiviral system in RAW 264.7 cells by Michael Litvack Download PDF EPUB FB2

DNA arrays are the most, common gene expression profiling technology. A DNA array consists of a solid support, (nylon membrane, glass chip) that carries DNA sequences representing genes - the probes. In hybridization experiments with the target sample of labeled complementary ribonucleic acids (cRNAs) and through subsequent data, capture a Cited by: “This beautifully illustrated book on gene expression profiling provides detailed protocols for a broad range of techniques for the analysis of gene expression at the DNA, RNA, and protein levels.

Although written for researchers, the book will be helpful for graduate students and others interested in learning about gene expression. Expression profiles of microRNAs in oxidized low-density lipoprotein-stimulated RAW cells Article in In Vitro Cellular & Developmental Biology - Animal 54(1) January with 26 Reads.

Objective To identify differentially expressed genes in peripheral blood mononuclear cells (PBMCs) from patients with ankylosing spondylitis (AS) compared with healthy individuals. Methods RNA was extracted from PBMCs collected from 18 patients with active disease and 18 gender-matched and age-matched controls.

Expression profiles of these cells were determined using by: Figure 2. Innate immune activation as measured by NF-κB activity and cytokine producing dendritic cells.

(a) NF-κB activation of RAW-Blue macrophage cell line. RAW-Blue cells were treated with each compound at μM for 18 h at 37 °C. Each figure is the result of six independent experiments, where *p Cited by: Human autoimmune diseases are well suited for the application of gene expression profiling.

Sampling of blood cells and target tissues has already revealed many important pathways contributing to this spectrum of disorders, and many commonalities are emerging.

Gene expression profiles in peripheral blood for the diagnosis of autoimmune diseases Bertalan Mesko1,3, Szilard Poliska1,3 and Laszlo Nagy1,2,3 1Department of Biochemistry and Molecular Biology, Research Center for Molecular Mediicne, Medical and Health Science Center, University of Debrecen, Debrecen, Hungary 2Apoptosis and Genomics Research Group of the Hungarian Academy of.

Differential gene expression profiling in blood from patients with digestive system cancers. of immune regulatory cells such as leukocytes and lymphocytes that are essential players in the host immune defense system.

These cells respond to various abnormal conditions such as viral. Gene expression analysis simultaneously compares the RNA expression levels of multiple genes (profiling) and/or multiple samples (screening).

This analysis can help scientists identify the molecular basis of phenotypic differences and to select gene expression targets for in-depth study. A broad range of Genotyping & Genomic Profiling products for analysis of genetic variation and genomic profiling, including in situ hybridization, SNP genotyping, AFLP and microsatellite analysis.

Bioinformatic analysis of microarray data was used to identify the regulatory patterns underlying changes in gene expression induced when RAW macrophages were stimulated via TLR9 by CpG.

The application of gene expression profiling to preneoplastic liver diseases and HCC is growing in importance and practicality. In this commentary, we review the recent advances in the utilization of global gene expression profiling to liver cancer, which have provided new insight into the molecular mechanisms underlying the development of HCC.

P.H. Muller, R. ten Cate, in Handbook of Systemic Autoimmune Diseases, Gene Expression Profiling. Gene expression profiling, also known as transcriptomics, measures the expression level of mRNAs (transcripts) in a cell population at a certain oligoarticular JIA, gene expression profiling on synovial fluid could help predict patients with an extended disease phenotype [60].

Exposure to RAW conditioned media, but not JA.1, strongly activated Luc expression in ISRE-Luc ovarian (7–13 fold increase) and breast (4 fold) cancer cells and not in the myeloma cells (–2 fold), supporting the hypothesis that soluble factors produced by RAW macrophages have activated the ISRE and expression of IFN.

Objective Gene expression profiling provides an opportunity to develop robust prognostic markers of colorectal carcinoma (CRC). However, the markers have not been applied for clinical decision making.

We aimed to develop an immunohistochemistry signature using microarray data for predicting CRC prognosis. Design We evaluated 25 CRC gene signatures in independent microarray datasets with. In the field of molecular biology, gene expression profiling is the measurement of the activity (the expression) of thousands of genes at once, to create a global picture of cellular profiles can, for example, distinguish between cells that are actively dividing, or show how the cells react to a particular treatment.

The strategy of gene-expression profiling was considered to be noninferior to the strategy of routine biopsies if the one-sided upper boundary of the 95% confidence interval for the hazard ratio.

The expression level of the target gene was standardised with that of the house-keeping beta-actin gene and the ratio of each gene expression in paired samples (adenoma or adenocarcinoma/adjacent. Prostate cancer, a leading cause of cancer death, displays a broad range of clinical behavior from relatively indolent to aggressive metastatic disease.

To explore potential molecular variation underlying this clinical heterogeneity, we profiled gene expression in 62 primary prostate tumors, as well as 41 normal prostate specimens and nine lymph node metastases, using cDNA. Mutations in the gene encoding emerin cause Emery-Dreifuss muscular dystrophy (EDMD), a disorder causing progressive skeletal muscle wasting, irregular heart rhythms and contractures of major tendons.

RNA sequencing was performed on differentiating wildtype and emerin-null myogenic progenitors to identify molecular pathways implicated in EDMD, genes were uniquely differentially expressed. The development of gene expression profiling is one of the most important achievements in cancer genetics in our time.

It is essentially the driving force behind personalized and precision medicine. This book highlights recent developments, applications, and breakthroughs in the field of gene expression profiling in cancer.Gene Expression Profiling of NFATc1-Knockdown in RAW Cells: An Alternative Pathway for Macrophage Differentiation Next Article in Special Issue CVm6A: A Visualization and Exploration Database for m 6 As in Cell Lines.

Gene therapy with viral vectors has progressed to clinical trials. However, the localization of viral vector delivery to diseased target sites remains a challenge. We tested the hypothesis that an.